bsm-52614R [Primary Antibody]
Angiotensin Converting Enzyme 2 Recombinant Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Angiotensin Converting Enzyme 2

Immunogen Range: 180-240/805


Clonality: Recombinant

Isotype: IgG

Entrez Gene: 59272

Swiss Prot: Q9BYF1

Source: C terminal human Angiotensin Converting Enzyme 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at -20°C for 12 months.

Background:

Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Endika Prieto-Fernndez. et al. Hypoxia reduces cell attachment of SARS-CoV-2 spike protein by modulating the expression of ACE2, neuropilin-1, syndecan-1 and cellular heparan sulfate. Emerg Microbes Infec. 2021;10(1):1065-1076Read more>>
VALIDATION IMAGES

IF(ICC) staining with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody (bsm-52614R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody (bsm-52614R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded human breast carcinoma tissue incubated with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody (bsm-52614R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody (bsm-52614R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody (bsm-52614R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody, Unconjugated (bsm-52614R) at 1:200 overnight at 4°C, DAB staining.