bsm-52623R
[Primary Antibody]
CD38 (10A10) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD38
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 952
Swiss Prot: P28907
Source: C terminal Human CD38
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: THP-1 Cells; Lane 2: Raji Cells; Probed with CD38 (10A10) Monoclonal Antibody (bsm-52623R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Ramos, 2: Raji Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 34kDa Observed MW: 45 kDa
Lane 1: Human MOLT4 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human Raji cell lysates probed with CD38 Monoclonal Antibody, Unconjugated (bsm-52623R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.