bsm-52639R [Primary Antibody]
Calnexin Recombinant Antibody
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Host: Rabbit

Target Protein: Calnexin

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 821

Swiss Prot: P27824

Source: C terminus human Calnexin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

Size: 100µL

Concentration: 1ug/ul

Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiaoyu Wang. et al. The key role of proteostasis at mitochondria-associated endoplasmic reticulum membrane in vanadium-induced nephrotoxicity using a proteomic strategy. SCI TOTAL ENVIRON. 2023 Apr;869:161741Read more>>
  • Zhenglin Li. et al. Cascaded microfluidic circuits for pulsatile filtration of extracellular vesicles from whole blood for early cancer diagnosis. SCI ADV. 2023 Apr;9(16)Read more>>
  • Wang Xiaoyu. et al. Toxic effects of copper on duck cerebrum: a crucial role of oxidative stress and endoplasmic reticulum quality control. ENVIRON SCI POLLUT R. 2023 Aug;:1-12Read more>>
  • Huang Qun. et al. Bushenhuoluo Decoction improves polycystic ovary syndrome by regulating exosomal miR-30a-5p/ SOCS3/mTOR/NLRP3 signaling-mediated autophagy and pyroptosis. J OVARIAN RES. 2024 Dec;17(1):1-17Read more>>
  • Li Yike. et al. Thermophoretic glycan profiling of extracellular vesicles for triple-negative breast cancer management. NAT COMMUN. 2024 Mar;15(1):1-15Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Calnexin Monoclonal Antibody, Unconjugated (bsm-52639R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Calnexin Monoclonal Antibody, Unconjugated (bsm-52639R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Calnexin Monoclonal Antibody, Unconjugated (bsm-52639R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Calnexin monoclonal antibody, unconjugated (bsm-52639R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.