bsm-52647R [Primary Antibody]
Tissue Factor (6A4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Tissue Factor

Immunogen Range: 1-100/295


Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2152

Swiss Prot: P13726

Source: C terminal Human Tissue Factor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Masahiro Terasawa. et al. Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium. Mar Drugs. 2022 Feb;20(2):121Read more>>
VALIDATION IMAGES

Lane 1: U937 Cells; Lane 2: SH-SY-5Y Cells; Lane 3: Mouse brain; Probed with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse pancreas tissue incubated with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Mouse Brain Lysates; Lane 2: Rat Kidney Lysates; Lane 3: Human A431 cell Lysates; Lane 4: Human MDA-MB-231 cell Lysates; Lane 5: Human U-87 MG cell Lysates; Lane 6: Human PANC-1 cell Lysates. Probed with Tissue factor monoclonal Antibody, unconjugated (bsm-52647R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.