VALIDATION IMAGES
Lane 1: U937 Cells; Lane 2: SH-SY-5Y Cells; Lane 3: Mouse brain; Probed with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse pancreas tissue incubated with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Mouse Brain Lysates; Lane 2: Rat Kidney Lysates; Lane 3: Human A431 cell Lysates; Lane 4: Human MDA-MB-231 cell Lysates; Lane 5: Human U-87 MG cell Lysates; Lane 6: Human PANC-1 cell Lysates. Probed with Tissue factor monoclonal Antibody, unconjugated (bsm-52647R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.