bsm-52651R

DATASHEET

Host: Rabbit

Target Protein: CDK4

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1019

Swiss Prot: P11802

Source: C terminal Human Cdk4

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Size: 100ul

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IF(ICC)(1:50-200)
IHC(1:50-200)
FCM(1:20-100)
IHC-P(1:200-400)

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.