DATASHEET
Host:
Rabbit
Target Protein:
IRF5
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
3663
Swiss Prot:
Q13568
Source:
N terminal human IRF5
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.
VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with IRF5 (2C5) Monoclonal Antibody (bsm-52655R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
THP-1 cells Probed with IRF5 (2C5) Monoclonal Antibody (bsm-52655R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with IRF5 (2C5) Monoclonal Antibody (bsm-52655R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.