VALIDATION IMAGES
Lane 1: PC-12 Cells; Lane 2: NIH/3T3 Cells; Probed with Cyclin A2 (2C2) Monoclonal Antibody (bsm-52660R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Cyclin A2 (2C2) Monoclonal Antibody (bsm-52660R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Cyclin A2 (2C2) Monoclonal Antibody (bsm-52660R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Cyclin A2 (2C2) Monoclonal Antibody (bsm-52660R) at 1:300 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Cyclin A2 (2C2) Monoclonal Antibody (bsm-52660R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Cyclin A2 (2C2) Monoclonal Antibody (bsm-52660R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Cyclin A2 (2C2) Monoclonal Antibody (bsm-52660R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Cyclin A2 (2C2) Monoclonal Antibody (bsm-52660R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Human HeLa cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human MOLT4 cell lysates; Lane 4: Human HL-60 cell lysates probed with CCNA2 Monoclonal Antibody, Unconjugated (bsm-52660R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.