bsm-52672R [Primary Antibody]
RUNX2 | CBFA1 Recombinant Antibody
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Host: Rabbit

Target Protein: RUNX2 | CBFA1

Immunogen Range: 300-450/521


Clonality: Recombinant

Isotype: IgG

Entrez Gene: 860

Swiss Prot: Q13950

Source: A synthesized peptide derived from human RUNX2

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:500-2000)
Flow-Cyt(1:100-200)
ICC/IF(1:100-200)

Predicted Molecular Weight: 57 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jia Gao. et al. Thymosin beta 10 loaded ZIF-8/sericin hydrogel promoting angiogenesis and osteogenesis for bone regeneration. INT J BIOL MACROMOL. 2024 May;267:131562Read more>>
  • Jingxi Xu. et al. Titanium dioxide nanoparticles oral exposure induce osteoblast apoptosis, inhibit osteogenic ability and increase lipogenesis in mouse. ECOTOX ENVIRON SAFE. 2024 Jun;277:116367Read more>>
VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with RUNX2 monoclonal antibody, unconjugated (bsm-52672R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


4% Paraformaldehyde-fixed MDA-MB-231 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RUNX2) monoclonal Antibody, unconjugated (bsm-52672R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.