bsm-52672R [Primary Antibody]
RUNX2 Recombinant Antibody
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Host: Rabbit

Target Protein: RUNX2

Immunogen Range: 300-450/521


Clonality: Recombinant

Isotype: IgG

Entrez Gene: 860

Swiss Prot: Q13950

Source: Human RUNX2 between 300 to 450 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 57


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jia Gao. et al. Thymosin beta 10 loaded ZIF-8/sericin hydrogel promoting angiogenesis and osteogenesis for bone regeneration. INT J BIOL MACROMOL. 2024 May;267:131562Read more>>
  • Jingxi Xu. et al. Titanium dioxide nanoparticles oral exposure induce osteoblast apoptosis, inhibit osteogenic ability and increase lipogenesis in mouse. ECOTOX ENVIRON SAFE. 2024 Jun;277:116367Read more>>
VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with RUNX2 monoclonal antibody, unconjugated (bsm-52672R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


The PC-3 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-RUNX2 antibody (bsm-52672R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488(bs-60295G-BF488): 1 μg/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.


4% Paraformaldehyde-fixed PC-3 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RUNX2) monoclonal Antibody, unconjugated (bsm-52672R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.