VALIDATION IMAGES
Lane 1: U937 Cells; Lane 2: Mouse spleen; Probed with RUNX2 (3C5) Monoclonal Antibody (bsm-52672R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with RUNX2 (3C5) Monoclonal Antibody (bsm-52672R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with RUNX2 (3C5) Monoclonal Antibody (bsm-52672R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: LnCAP (Negative control), 2: MDA-MB-231, 3: PC-3 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 57 kDa Observed MW: 64 kDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Saos-2, 2: C2C12 Protein loading quantity: 20 μg Exposure time: 30 s / 60 s Predicted MW: 57 kDa Observed MW: 64 kDa
Lane 1: Human MDA-MB-231 cell lysates; Lane 2: Human U251 cell lysates probed with RUNX2 Monoclonal Antibody, Unconjugated (bsm-52672R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.