bsm-52672R [Primary Antibody]
RUNX2 (3C5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RUNX2

Immunogen Range: 300-450/521


Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 860

Swiss Prot: Q13950

Source: Human RUNX2 between 300 to 450 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: U937 Cells; Lane 2: Mouse spleen; Probed with RUNX2 (3C5) Monoclonal Antibody (bsm-52672R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with RUNX2 (3C5) Monoclonal Antibody (bsm-52672R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with RUNX2 (3C5) Monoclonal Antibody (bsm-52672R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: LnCAP (Negative control), 2: MDA-MB-231, 3: PC-3 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 57 kDa Observed MW: 64 kDa


Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Saos-2, 2: C2C12 Protein loading quantity: 20 μg Exposure time: 30 s / 60 s Predicted MW: 57 kDa Observed MW: 64 kDa


Lane 1: Human MDA-MB-231 cell lysates; Lane 2: Human U251 cell lysates probed with RUNX2 Monoclonal Antibody, Unconjugated (bsm-52672R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.