bsm-52675R
[Primary Antibody]
TRAF2 (9C2) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: TRAF2
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 7186
Swiss Prot: Q12933
Source: Human TRAF2 aa 1-100
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with TRAF2 (9C2) Monoclonal Antibody (bsm-52675R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Hela cells probed with TRAF2 (9C2) Monoclonal Antibody (bsm-52675R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with TRAF2 (9C2) Monoclonal Antibody (bsm-52675R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with TRAF2 (9C2) Monoclonal Antibody (bsm-52675R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse placenta tissue incubated with TRAF2 (9C2) Monoclonal Antibody (bsm-52675R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.