bsm-52694R [Primary Antibody]
Hsp20 (7C5) Monoclonal Antibody
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Host: Rabbit

Target Protein: Hsp20

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 126393

Swiss Prot: O14558

Source: Recombinant human Hsp20 between 50-150 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Seems to have versatile functions in various biological processes. Plays a role in regulating muscle function such as smooth muscle vasorelaxation and cardiac myocyte contractility. May regulate myocardial angiogenesis implicating KDR. Overexpression mediates cardioprotection and angiogenesis after induced damage. Stabilizes monomeric YWHAZ thereby supporting YWHAZ chaperone-like activity.

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
IHC

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of HeLa cells with Hsp20 (7C5) Monoclonal Antibody (bsm-52694R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: Human skeletal muscle; Lane 2: Mouse skeletal muscle; Probed with Hsp20 (7C5) Monoclonal Antibody (bsm-52694R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Mouse skeletal muscle tissue incubated with Hsp20 (7C5) Monoclonal Antibody (bsm-52694R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with Hsp20 (7C5) Monoclonal Antibody (bsm-52694R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.