bsm-52695R [Primary Antibody]
Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Ubiquitin-like modifier-activating enzyme 1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7317

Swiss Prot: P22314

Source: Human Ubiquitin-like modifier-activating enzyme 1 between 1000 amino acids to C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
IHC

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse liver lysate probed with Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody (bsm-52695R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody (bsm-52695R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody (bsm-52695R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody (bsm-52695R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse ovary tissue incubated with Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody (bsm-52695R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.