DATASHEET
Host:
Rabbit
Target Protein:
Cdc6 (Ser54)
Modification Site:
Ser54
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
990
Swiss Prot:
Q99741
Source:
Synthetic phospho-peptide surrounding Ser54 of human Cdc6
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
VALIDATION IMAGES
Jurkat cells Probed with Phospho-Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.