bsm-52708R [Primary Antibody]
Cdc6 (Ser54) (6F5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cdc6 (Ser54)

Modification Site: Ser54

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 990

Swiss Prot: Q99741

Source: Synthetic phospho-peptide surrounding Ser54 of human Cdc6

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Jurkat cells Probed with Phospho-Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Cdc6 (Ser54) (6F5) Monoclonal Antibody (bsm-52708R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.