bsm-52710R [Primary Antibody]
14-3-3 alpha+beta (3C5) Monoclonal Antibody
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Host: Rabbit

Target Protein: 14-3-3 alpha+beta

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7529

Swiss Prot: P31946

Source: Synthetic peptide corresponding to C terminal human 14-3-3 alpha + beta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.

Size: 100uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
IHC(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.