Host: Rabbit
Target Protein: Hsp22
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 26353
Swiss Prot: Q9UJY1
Source: Recombinant protein within Human Hsp20 aa 50-150
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: HepG2 Cells; Lane 2: Mouse skeletal muscle; Probed with Hsp22 (5C5) Monoclonal Antibody (bsm-52712R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Hsp22 (5C5) Monoclonal Antibody (bsm-52712R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Hsp22 (5C5) Monoclonal Antibody (bsm-52712R) at 1:300 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Hsp22 (5C5) Monoclonal Antibody (bsm-52712R) at 1:300 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat skeletal muscle tissue incubated with Hsp22 (5C5) Monoclonal Antibody (bsm-52712R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.