VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with IL1 beta (1T3) Monoclonal Antibody (bsm-52727R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with IL1 beta (1T3) Monoclonal Antibody (bsm-52727R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with IL1 beta (1T3) Monoclonal Antibody (bsm-52727R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with IL1 beta (1T3) Monoclonal Antibody (bsm-52727R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Human THP-1 cell lysates; Lane 2: Human THP-1 treated with 80nM TPA for 24h, then treated with 100ng/ml lipopolysaccharide (LPS) for 6h, then with 300ng/ml Brefeldin A added after 3 hours probed with IL-1 beta Monoclonal Antibody, Unconjugated (bsm-52727R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.