bsm-52749R [Primary Antibody]
SUZ12 (3C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SUZ12

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 23512

Swiss Prot: Q15022

Source: Human SUZ12 between 50 to 150 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

MCF-7 cell lysate probed with SUZ12 (3C1) Monoclonal Antibody (bsm-52749R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with SUZ12 (3C1) Monoclonal Antibody (bsm-52749R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with SUZ12 (3C1) Monoclonal Antibody (bsm-52749R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with SUZ12 (3C1) Monoclonal Antibody (bsm-52749R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Bone lysates; Lane 3: Mouse Uterus lysates; Lane 4: RAT Liver cell lysates; Lane 5: Human JAR cell lysates; Lane 6: Human MDA-MB-231 cell lysates; Lane 7: Human SH-SY5Y cell lysates probed with SUZ12 (3C1) Monoclonal Antibody, Unconjugated (bsm-52749R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.