VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with Hsp27 (7C4) Monoclonal Antibody (bsm-52757R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
A549 cell lysate probed with Hsp27 (7C4) Monoclonal Antibody (bsm-52757R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Hsp27 (7C4) Monoclonal Antibody (bsm-52757R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Hsp27 (7C4) Monoclonal Antibody (bsm-52757R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Hsp27 (7C4) Monoclonal Antibody (bsm-52757R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Hsp27 (7C4) Monoclonal Antibody (bsm-52757R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Hsp27 (7C4) Monoclonal Antibody (bsm-52757R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.