bsm-52760R
[Primary Antibody]
E2F1 (3C2) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: E2F1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 1869
Swiss Prot: Q01094
Source: Recombinant human E2F1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with E2F1 (3C2) Monoclonal Antibody (bsm-52760R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
HepG2 cell lysate probed with E2F1 (3C2) Monoclonal Antibody (bsm-52760R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with E2F1 (3C2) Monoclonal Antibody (bsm-52760R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with E2F1 (3C2) Monoclonal Antibody (bsm-52760R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with E2F1 (3C2) Monoclonal Antibody (bsm-52760R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with E2F1 (3C2) Monoclonal Antibody (bsm-52760R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse pancreas tissue incubated with E2F1 (3C2) Monoclonal Antibody (bsm-52760R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.