bsm-52762R
[Primary Antibody]
CDC5L (6C8) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CDC5L
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 988
Swiss Prot: Q99459
Source: Human CDC5L aa 200-400
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Hela Cells; Lane 2: Jurkat Cells; Probed with CDC5L (6C8) Monoclonal Antibody (bsm-52762R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with CDC5L (6C8) Monoclonal Antibody (bsm-52762R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CDC5L (6C8) Monoclonal Antibody (bsm-52762R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CDC5L (6C8) Monoclonal Antibody (bsm-52762R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CDC5L (6C8) Monoclonal Antibody (bsm-52762R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with CDC5L (6C8) Monoclonal Antibody (bsm-52762R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with CDC5L (6C8) Monoclonal Antibody (bsm-52762R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.