bsm-52779R
[Primary Antibody]
PTP1B (4C3) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: PTP1B
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 5770
Swiss Prot: P18031
Source: Human PTP1B aa 40-250
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Zebrafish
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of raji cells with PTP1B (4C3) Monoclonal Antibody (bsm-52779R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Zebra fish Cells; Lane 2: MCF-7 Cells; Lane 3: HepG2 Cells; Lane 4: A431 Cells; Probed with PTP1B (4C3) Monoclonal Antibody (bsm-52779R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with PTP1B (4C3) Monoclonal Antibody (bsm-52779R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PTP1B (4C3) Monoclonal Antibody (bsm-52779R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PTP1B (4C3) Monoclonal Antibody (bsm-52779R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with PTP1B (4C3) Monoclonal Antibody (bsm-52779R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.