bsm-52784R
[Primary Antibody]
EBP50 (3C3) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: EBP50
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 9368
Swiss Prot: O14745
Source: C terminal human EBP50
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
MCF-7 cells Probed with EBP50 (3C3) Monoclonal Antibody (bsm-52784R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Rat kidney tissue incubated with EBP50 (3C3) Monoclonal Antibody (bsm-52784R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with EBP50 (3C3) Monoclonal Antibody (bsm-52784R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.