VALIDATION IMAGES
Lane 1: Human lung lysates; Lane 2: HepG2 lysates; Line 3: 293T lysates; Probed with Mannose Receptor(CD206) (1C4) Monoclonal Antibody (bsm-52791R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Mannose Receptor(CD206) (1C4) Monoclonal Antibody (bsm-52791R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Mannose Receptor(CD206) (1C4) Monoclonal Antibody (bsm-52791R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Lane 1: Human HepG2 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human 293T cell lysates; Lane 4: Human MCF-7 cell lysates ; Lane 5: Human Molt-4 cell lysates probed with Mannose Receptor(CD206) (1C4) Monoclonal Antibody, Unconjugated (bsm-52791R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.