bsm-52808R [Primary Antibody]
PDIA6 (4C3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PDIA6

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10130

Swiss Prot: Q15084

Source: Human PDIA6 between 300 to 500 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

May function as a chaperone that inhibits aggregation of misfolded proteins (PubMed:12204115). Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling (PubMed:24508390). May also regulate the UPR via the EIF2AK3 UPR sensor (PubMed:24508390). Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (PubMed:15466936).

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of HeLa cells with PDIA6 (4C3) Monoclonal Antibody (bsm-52808R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: HepG2 lysates; Lane 2: K562 lysates; Probed with PDIA6 (4C3) Monoclonal Antibody (bsm-52808R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with PDIA6 (4C3) Monoclonal Antibody (bsm-52808R) at 1:300 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with PDIA6 (4C3) Monoclonal Antibody (bsm-52808R) at 1:300 in NIH/3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.