VALIDATION IMAGES
Flow cytometric analysis of sh-sy5ycells with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: A431 lysates; Lane 2: Mouse lung lysates; Probed with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:100 in HeLa cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:300 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:300 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human stomach carcinoma tissue incubated with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.