bsm-52813R [Primary Antibody]
Thymidine Kinase 1 (9C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Thymidine Kinase 1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7083

Swiss Prot: P04183

Source: Human Thymidine Kinase 1 around C terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of HeLa cells with Thymidine Kinase 1 (9C1) Monoclonal Antibody (bsm-52813R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: 293T lysates; Lane 2: Hela lysates; Probed with Thymidine Kinase 1 (9C1)Monoclonal Antibody (bsm-52813R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Thymidine Kinase 1 (9C1) Monoclonal Antibody (bsm-52813R) at 1:100 in NIH/3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Thymidine Kinase 1 (9C1) Monoclonal Antibody (bsm-52813R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.