DATASHEET
Host:
Rabbit
Target Protein:
Thymidine Kinase 1
Clonality:
Recombinant
Isotype:
IgG
Entrez Gene:
7083
Swiss Prot:
P04183
Source:
Human Thymidine Kinase 1 around C terminus
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage:
Store at -20°C for 12 months.
Background:
Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
VALIDATION IMAGES
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Thymidine Kinase 1 (9C1) Monoclonal Antibody (bsm-52813R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
25 ug total protein per lane of various lysates (see on figure) probed with Thymidine kinase 1 monoclonal antibody, unconjugated (bsm-52813R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.