bsm-52824R
[Primary Antibody]
NCAM (8C3) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: NCAM
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 4684
Swiss Prot: P13591
Source: C terminal human NCAM
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Zebrafish
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhang Yufan. et al. Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering. J NANOBIOTECHNOL. 2022 Dec;20(1):1-22Read more>>
VALIDATION IMAGES
Flow cytometric analysis of sh-sy5ycells with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: SH-SY-5Y lysates; Lane 2: Human brain lysates; Probed with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100 in A549 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100 in N2A cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Zebra fish kidney tissue incubated with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: The room temperature was 2 h Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: SH-SY5Y, 2: Mouse cerebellum, 3: Rat cerebellum Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 95 kDa Observed MW:140-180 kDa
Cell line: SH-SY5Y Fixative: 4% Paraformaldehyde Permeabilization: 90% methanol Primary ab dilution: 1:100 Secondary ab: Goat Anti-Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for PTM-6218
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NCAM) polyclonal Antibody, Unconjugated (bsm-52824R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.