bsm-52825R
[Primary Antibody]
Dysferlin (7C1) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Dysferlin
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 8291
Swiss Prot: O75923
Source: Human Dysferlin aa 100-200
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity).
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Human skeletal muscle lysates probed with Dysferlin (7C1) Monoclonal Antibody (bsm-52825R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Dysferlin (7C1) Monoclonal Antibody (bsm-52825R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse skeletal muscle tissue incubated with Dysferlin (7C1) Monoclonal Antibody (bsm-52825R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with Dysferlin (7C1) Monoclonal Antibody (bsm-52825R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse spleen tissue incubated with Dysferlin (7C1) Monoclonal Antibody (bsm-52825R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.