bsm-52826R
[Primary Antibody]
CNPase (1C9) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CNPase
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 1267
Swiss Prot: P09543
Source: Human CNPase aa 1-200
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of sh-sy5y cells with yCNPase (1C9) Monoclonal Antibody (bsm-52826R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Mouse brain lysates probed with CNPase (1C9) Monoclonal Antibody (bsm-52826R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with CNPase (1C9) Monoclonal Antibody (bsm-52826R) at 1:100 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CNPase (1C9) Monoclonal Antibody (bsm-52826R) at 1:100 in N2A cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with CNPase (1C9) Monoclonal Antibody (bsm-52826R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.