Host: Rabbit
Target Protein: MHC class I
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 3105
Swiss Prot: P30443
Source: Human MHC class I aa 1-200
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Raji lysates; Lane 2: THP-1 lysates; Probed with MHC class I (10G4)Monoclonal Antibody (bsm-52827R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with MHC class I (10G4) Monoclonal Antibody (bsm-52827R) at 1:300 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with MHC class I (10G4) Monoclonal Antibody (bsm-52827R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Human A431 cell lysates; Lane 2: Human U937 cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human THP-1 cell lysates probed with MHC class I Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MHC class I) Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MHC class I) Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U266 cell lysates probed with MHC class I Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.