bsm-52828R
[Primary Antibody]
Galectin 3 (2C7) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Galectin 3
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 3958
Swiss Prot: P17931
Source: Human Galectin 3 aa 150-250
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with Galectin 3 (2C7) Monoclonal Antibody (bsm-52828R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela lysates; Lane 2: NIH/3T3 lysates; Line 3: THP-1 lysates ;Probed with Galectin 3 (2C7) Monoclonal Antibody (bsm-52828R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Galectin 3 (2C7) Monoclonal Antibody (bsm-52828R) at 1:100 in NIH/3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.