VALIDATION IMAGES
Lane 1: Hela lysates; Lane 2: Jurkat lysates; Line 3: Mouse kidney lysates ;Probed with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100 in HeLa cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100 in SKOV-3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Eye lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Rat Liver lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human MCF-7 cell lysates probed with CD147 Monoclonal Antibody, Unconjugated (bsm-52837R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.