bsm-52837R [Primary Antibody]
CD147 Recombinant Antibody
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Host: Rabbit

Target Protein: CD147

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 682

Swiss Prot: P35613

Source: Human CD147 aa 100-250

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at -20°C for 12 months.

Background:

Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.

Size: 100µL

Concentration: 1ug/ul

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xuejiao Zhu. et al. Basigin-CyP elevated porcine circovirus type2 replication. Virus Res. 2020 Nov;289:198152Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Eye lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Rat Liver lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human MCF-7 cell lysates probed with CD147 Monoclonal Antibody, Unconjugated (bsm-52837R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD147 Monoclonal Antibody, Unconjugated (bsm-52837R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD147 Monoclonal Antibody, Unconjugated (bsm-52837R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD147 Monoclonal Antibody, Unconjugated (bsm-52837R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.