DATASHEET
Host:
Rabbit
Target Protein:
Annexin V
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
308
Swiss Prot:
P08758
Source:
Human Annexin V aa 1-150
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with Annexin V (6C7) Monoclonal Antibody (bsm-52841R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela lysates; Lane 2: HepG2 lysates; Line 3: JAR lysates; Probed with Annexin V (6C7) Monoclonal Antibody (bsm-52841R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Annexin V (6C7) Monoclonal Antibody (bsm-52841R) at 1:300 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.