bsm-52848R
[Primary Antibody]
GFP (HRP conjugated) (14C4) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: GFP
Clonality: Monoclonal
Isotype: IgG
Source: Recombinant protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
All
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
K562 cell lysate transfected with GFP probed with GFP (HRP conjugated) (14C4) Monoclonal Antibody (bsm-52848R) at 1:1000 overnight at 4°C.