bsm-52858R
[Primary Antibody]
ASPP2 (2C3) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ASPP2
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 7159
Swiss Prot: Q13625
Source: Human ASPP2 aa 1-150
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Hela lysates; Lane 2: MCF-7 lysates; Probed with ASPP2 (2C3)Thioredoxin (4C7) Monoclonal Antibody (bsm-52858R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with ASPP2 (2C3) Monoclonal Antibody (bsm-52858R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with ASPP2 (2C3) Monoclonal Antibody (bsm-52858R) at 1:100 in 293T cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.