bsm-52868R
[Primary Antibody]
Thioredoxin (9C1) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Thioredoxin
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 7295
Swiss Prot: P10599
Source: Human Thioredoxin aa 50-150
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with Thioredoxin (9C1) Monoclonal Antibody (bsm-52868R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela lysates; Lane 2: 293T lysates; Probed with Thioredoxin (9C1) Monoclonal Antibody (bsm-52868R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.