bsm-52871R
[Primary Antibody]
Ras (2C4) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Ras
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 4893
Swiss Prot: P01111
Source: Human Ras aa 1-100
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
N-RAS (neuroblastoma RAS viral oncogene homolog) analogously to other GTP-binding proteins (such as Translation Elongation Factor EFTu or signal transducing G-Proteins) RAS proteins are involved in signal transduction pathways, ras gene family is part of the ras superfamily including the mammalian RAS, RAL, RAC, RHO, RAP, and RAB gene families and the yeast homologs like SEC4 and YPT1 genes; genes encode small monomeric proteins of low molecular mass (20-30 kDa) which share at least 30% homology to RAS proteins.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
Zebrafish
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: 293T lysates; Lane 2: MCF-7 lysates; Lane 3: Hela lysates; Lane 4: Zebra fish lysates; Probed with Ras (2C4) Monoclonal Antibody (bsm-52871R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Ras (2C4) Monoclonal Antibody (bsm-52871R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Ras (2C4) Monoclonal Antibody (bsm-52871R) at 1:200 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.