bsm-52884R [Primary Antibody]
Vitamin D Binding protein (56C9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Vitamin D Binding protein

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2638

Swiss Prot: P02774

Source: Vitamin D Binding protein aa 250-350

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Vitamin D Binding Protein is a secreted multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and urine, and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane bound immunoglobulin on the surface of B lymphocytes and with IgG fc receptor on the membranes of T lymphocytes.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of HepG2 cells with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Human lung lysates probed with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:100 in SKOV-3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Vitamin D Binding protein (56C9) Monoclonal Antibody (bsm-52884R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.