bsm-52903R
[Primary Antibody]
CD59 (1C7) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD59
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 966
Swiss Prot: P13987
Source: extracellular domain of Human CD59
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of nih-3t3 cells with CD59 (1C7) Monoclonal Antibody (bsm-52903R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Human placenta cells lysates; Lane 2: HUVEC cells lysates; Line 3: K562 cells lysates ;Probed with CD59 (1C7) Monoclonal Antibody (bsm-52903R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with CD59 (1C7) Monoclonal Antibody (bsm-52903R) at 1:200 in JAR cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CD59 (1C7) Monoclonal Antibody (bsm-52903R) at 1:200 in NIH-3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.