bsm-52904R-BF350

DATASHEET

Host: Rabbit

Target Protein: ATPB

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 506

Swiss Prot: P06576

Source: Human ATPB between 100 to 250 amino aids

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Conjugation: AbBy Fluor-350

Excitation/ Emission: 346nm/442nm

Size: 100µL

Concentration: 1mg/ml

Applications: WB(WB(1:1000-2000))
IF(IHC-P)(IF(IHC-P)(1:200-500))
IF(ICC)(IF(ICC)(1:100-200))

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.