bsm-52909R
[Primary Antibody]
AQP1 (9C8) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: AQP1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 358
Swiss Prot: P29972
Source: Human AQP1 aa 50-200
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Hela cells lysates; Lane 2: Jurkat cells lysates; Line 3: Human kidney cells lysates ;Probed with AQP1 (9C8) Monoclonal Antibody (bsm-52909R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with AQP1 (9C8) Monoclonal Antibody (bsm-52909R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with AQP1 (9C8) Monoclonal Antibody (bsm-52909R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with AQP1 (9C8) Monoclonal Antibody (bsm-52909R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human breast tissue incubated with AQP1 (9C8) Monoclonal Antibody (bsm-52909R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with AQP1 (9C8) Monoclonal Antibody (bsm-52909R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with AQP1 (9C8) Monoclonal Antibody (bsm-52909R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with AQP1 (9C8) Monoclonal Antibody (bsm-52909R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with AQP1 (9C8) Monoclonal Antibody (bsm-52909R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.