bsm-52915R
[Primary Antibody]
SDHA (2C2) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: SDHA
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 6389
Swiss Prot: P31040
Source: Human SDHA aa 550-650
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
Zebrafish
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of Hela cells with SDHA (2C2) Monoclonal Antibody (bsm-52915R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black)
Lane 1: Jurkat cells lysates; Probed with SDHA (2C2)Monoclonal Antibody (bsm-52915R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with SDHA (2C2) Monoclonal Antibody (bsm-52915R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with SDHA (2C2) Monoclonal Antibody (bsm-52915R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with SDHA (2C2) Monoclonal Antibody (bsm-52915R) at 1:100 in NIH-3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with SDHA (2C2) Monoclonal Antibody (bsm-52915R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with SDHA (2C2) Monoclonal Antibody (bsm-52915R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with SDHA (2C2) Monoclonal Antibody (bsm-52915R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Zebrafish tissue incubated with SDHA (2C2) Monoclonal Antibody (bsm-52915R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.