VALIDATION IMAGES
A431 cells lysates probed with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
Paraformaldehyde-fixed and paraffin-embedded Zebrafish tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in NIH-3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed, paraffin embedded Human kidney tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD73 (2C9) Monoclonal Antibody, Unconjugated (bsm-52916R) at 1:200 overnight at 4°C, DAB staining.
IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed, paraffin embedded Human endometrial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD73 (2C9) Monoclonal Antibody, Unconjugated (bsm-52916R) at 1:200 overnight at 4°C, DAB staining.