bsm-52916R [Primary Antibody]
CD73 (2C9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD73

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4907

Swiss Prot: P21589

Source: Human CD73 between 1 to 100 amino acids.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

A431 cells lysates probed with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin


Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin


Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin


Paraformaldehyde-fixed and paraffin-embedded Zebrafish tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin


IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in NIH-3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed, paraffin embedded Human kidney tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD73 (2C9) Monoclonal Antibody, Unconjugated (bsm-52916R) at 1:200 overnight at 4°C, DAB staining.


IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed, paraffin embedded Human endometrial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD73 (2C9) Monoclonal Antibody, Unconjugated (bsm-52916R) at 1:200 overnight at 4°C, DAB staining.