bsm-52920R [Primary Antibody]
DRD1 (3C3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DRD1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1812

Swiss Prot: P21728

Source: Human Dopamine Receptor D1 between 400 to C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Carpenter, Marco D.. et al. Cell-type specific profiling of histone post-translational modifications in the adult mouse striatum. NAT COMMUN. 2022 Dec;13(1):1-12Read more>>
VALIDATION IMAGES

Mouse kidney cells lysates probed with DRD1 (3C3) Monoclonal Antibody (bsm-52920R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with DRD1 (3C3) Monoclonal Antibody (bsm-52920R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with DRD1 (3C3) Monoclonal Antibody (bsm-52920R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


IF(ICC) staining with DRD1 (3C3) Monoclonal Antibody (bsm-52920R) at 1:200 in 293T cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with DRD1 (3C3) Monoclonal Antibody (bsm-52920R) at 1:200 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine Receptor D1) Monoclonal Antibody, Unconjugated (bsm-52920R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Lane 1: Human SH-SY5Y cell lysates probed with DRD1 Monoclonal Antibody, Unconjugated (bsm-52920R) at 1:200 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.