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Host: Rabbit
Target Protein: CD171/L1CAM
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 3897
Swiss Prot: P32004
Source: A synthesized peptide derived from human NCAM L1
Purification: Purified by Protein A.
Storage Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:1000-5000))
IHC-P(IHC-P(1:200-800))
IHC-F(IHC-F(1:200-800))
IF(IHC-P)(IF(IHC-P)(1:200-800))
Predicted Molecular Weight: 138
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD171/L1CAM Monoclonal Antibody, Unconjugated(bsm-52926R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD171/L1CAM Monoclonal Antibody, Unconjugated(bsm-52926R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD171/L1CAM Monoclonal Antibody, Unconjugated(bsm-52926R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with CD171/L1CAM monoclonal antibody, unconjugated (bsm-52926R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD171/L1CAM Monoclonal Antibody, Unconjugated (bsm-52926R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.