bsm-52938R [Primary Antibody]
TNF Receptor II (7D3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TNF Receptor II

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7133

Swiss Prot: P20333

Source: C terminus Human TNF Receptor II

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of HL-60 cells with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black)


Lane 1: MCF-7 cells lysates; Lane 2: Jurkat cells lysates; Probed with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Human THP-1 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human U937 cell; Lane 4: Human U87MG cell；Lane 5: Human HUVEC cell. Probed with TNFR2 monoclonal Antibody, unconjugated (bsm-52938R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.