bsm-52939R
[Primary Antibody]
CD40L (4C2) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD40L
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 959
Swiss Prot: P29965
Source: N terminus human CD40L
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD4L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of thp-1 cells with CD40L (4C2) Monoclonal Antibody(bsm-52939R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela cells lysates; Lane 2: Mouse liver cells lysates; Probed with CD40L (4C2) Monoclonal Antibody (bsm-52939R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with CD40L (4C2) Monoclonal Antibody (bsm-52939R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CD40L (4C2) Monoclonal Antibody (bsm-52939R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.