VALIDATION IMAGES
Lane 1: Hela cells lysates; Lane 2: K563 cells lysates; Probed with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of HeLa cells with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:200 in SH-SY5Y cells (red) . The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.