bsm-52949R [Primary Antibody]
TDP43 (6C6) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TDP43

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 23435

Swiss Prot: Q13148

Source: Human TDP43 between 250-350 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cells lysates; Lane 2: K563 cells lysates; Probed with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of HeLa cells with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


IF(ICC) staining with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:200 in SH-SY5Y cells (red) . The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with TDP43 (6C6) Monoclonal Antibody (bsm-52949R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.