bsm-52962R [Primary Antibody]
POLR2A (Ser5) (12C8) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: POLR2A (Ser5)

Modification Site: Ser5

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5430

Swiss Prot: P24928

Source: Synthetic phospho-peptide corresponding to residues surrounding Ser5 of human POLR2A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cells lysates; Lane 2: MCF-7 cells lysates; Probed with POLR2A (Ser5) (12C8) Monoclonal Antibody (bsm-52962R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of Hela cells with POLR2A (Ser5) (12C8) Monoclonal Antibody (bsm-52962R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Phospho-POLR2A (S5) (12C8) Monoclonal Antibody (bsm-52962R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Phospho-POLR2A (S5) (12C8) Monoclonal Antibody (bsm-52962R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human breast cancer tissue incubated with Phospho-POLR2A (S5) (12C8) Monoclonal Antibody (bsm-52962R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse bladder tissue incubated with Phospho-POLR2A (S5) (12C8) Monoclonal Antibody (bsm-52962R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


IF(ICC) staining with Phospho-POLR2A (S5) (12C8) Monoclonal Antibody (bsm-52962R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Phospho-POLR2A (S5) (12C8) Monoclonal Antibody (bsm-52962R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Phospho-POLR2A (S5) (12C8) Monoclonal Antibody (bsm-52962R) at 1:200 in PC-12 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Phospho-POLR2A (S5) (12C8) Monoclonal Antibody (bsm-52962R) at 1:200 in PC-12 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.