bsm-52964R
[Primary Antibody]
MIF (8C2) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MIF
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 4282
Swiss Prot: P14174
Source: C terminal human MIF
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Mouse brain cells lysates; Lane 2: Jurkat cells lysates; Line 3: Hela cells lysates;Probed with MIF (8C2) Monoclonal Antibody (bsm-52964R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.