bsm-52978R [Primary Antibody]
FGF21 Monoclonal Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FGF21

Immunogen Range: 1-100/208


Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 26291

Swiss Prot: Q9NSA1

Source: Human FGF21

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(WB(1:1000-5000))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(bsm-52978R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(bsm-52978R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(bsm-52978R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(bsm-52978R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(bsm-52978R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(bsm-52978R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated(bsm-52978R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.


25 ug total protein per lane of various lysates (see on figure) probed with FGF21 monoclonal antibody, unconjugated (bsm-52978R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FGF21 Monoclonal Antibody, Unconjugated (bsm-52978R) at 1:200 overnight at 4°C, followed by conjugation to the PV-1023 and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FGF21 Monoclonal Antibody, Unconjugated (bsm-52978R) at 1:200 overnight at 4°C, followed by conjugation to the PV-1023 and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FGF21 Monoclonal Antibody, Unconjugated (bsm-52978R) at 1:200 overnight at 4°C, followed by conjugation to the PV-1023 and DAB (C-0010) staining.